synthetic gene fragment Search Results


synthetic gene fragment  (GenScript corporation)
90
GenScript corporation synthetic gene fragment
Synthetic Gene Fragment, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragment - by Bioz Stars, 2026-11
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sequence of the synthetic gene fragment  (Entelechon GmbH)
90
Entelechon GmbH sequence of the synthetic gene fragment
Sequence Of The Synthetic Gene Fragment, supplied by Entelechon GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragments tad c 3x to tad v 3x  (GenScript corporation)
90
GenScript corporation synthetic gene fragments tad c 3x to tad v 3x
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragments Tad C 3x To Tad V 3x, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
synthetic gene fragments tad c 3x to tad v 3x - by Bioz Stars, 2026-11
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synthetic gblockstm gene fragments  (DNA Technologies Inc)
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DNA Technologies Inc synthetic gblockstm gene fragments
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gblockstm Gene Fragments, supplied by DNA Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/synthetic+gene+fragment/synthetic+gblockstm+gene+fragments/pmc11349219-163-5-10
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synthetic gene fragment of vps5  (GenScript corporation)
90
GenScript corporation synthetic gene fragment of vps5
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragment Of Vps5, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragments for s gene  (Xcelris Genomics)
90
Xcelris Genomics synthetic gene fragments for s gene
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragments For S Gene, supplied by Xcelris Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragments for s gene - by Bioz Stars, 2026-11
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synthetic fragment encoding the mc159l gene of molluscum contagiosum virus subtype 1  (GenScript corporation)
90
GenScript corporation synthetic fragment encoding the mc159l gene of molluscum contagiosum virus subtype 1
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Fragment Encoding The Mc159l Gene Of Molluscum Contagiosum Virus Subtype 1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragment encoding the extracellular region of a metagenomic fsx1 orf  (GenScript corporation)
90
GenScript corporation synthetic gene fragment encoding the extracellular region of a metagenomic fsx1 orf
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragment Encoding The Extracellular Region Of A Metagenomic Fsx1 Orf, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragment of rnf219 codon optimized for expression in s. frugiperda  (GenScript corporation)
90
GenScript corporation synthetic gene fragment of rnf219 codon optimized for expression in s. frugiperda
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragment Of Rnf219 Codon Optimized For Expression In S. Frugiperda, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragment of rnf219 codon optimized for expression in s. frugiperda - by Bioz Stars, 2026-11
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synthetic gene fragments  (Microsynth ag)
90
Microsynth ag synthetic gene fragments
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragments, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic gene fragments encoding vh and vl of the anti-14e06 monoclonal antibody 2g91  (GenScript corporation)
90
GenScript corporation synthetic gene fragments encoding vh and vl of the anti-14e06 monoclonal antibody 2g91
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Gene Fragments Encoding Vh And Vl Of The Anti 14e06 Monoclonal Antibody 2g91, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dna fragment of the aatf gene  (GenScript corporation)
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GenScript corporation synthetic dna fragment of the aatf gene
Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing <t>synthetic</t> proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic <t>gene</t> <t>fragments</t> is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="250" height="auto" />
Synthetic Dna Fragment Of The Aatf Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing synthetic proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic gene fragments is shown in <xref ref-type=Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol. " width="100%" height="100%">

Journal: The Journal of Biological Chemistry

Article Title: Synthetic transcription factors establish the function of nine amino acid transactivation domains of Komagataella phaffii Mxr1

doi: 10.1016/j.jbc.2025.108211

Figure Lengend Snippet: Identification of Mxr1 9aaTADs that are functional during methanol metabolism. A , strategy for the construction of Komagataella phaffii strains expressing synthetic proteins harboring Mxr1 DBD and three copies of 20 putative TADs (Mxr1ZF-TAD C-V 3x ). This figure was created using BioRender (, https://BioRender.com/s96u589 ). The nucleotide sequence of synthetic gene fragments is shown in Figure S2 . B , schematic diagram of Mxr1ZF-TAD C-V 3x . C , protein profile of K. phaffii strains cultured in BMMY medium as analyzed by SDS-PAGE followed by Coomassie Brilliant Blue R staining. M, protein molecular mass markers (kilodalton). DHAS and AOX proteins are restored in K. phaffii strains expressing functional 9aaTADs (TADs A, G, H, I, N, O, Q, R, S, T, and U). These are underlined. D , quantification of AOX1, DHAS1, FLD , and FDH mRNAs in different K. phaffii strains cultured in YNBM for 6 h by qPCR. Error bars indicate mean ± SD (n = 3). Individual data points indicate the three biological replicates. K. phaffii strains in which these mRNA levels are comparable to that of GS115 are underlined. E , analysis of growth of GS115 , Δmxr1 , and K. phaffii strains in which 9aaTADs are functional (TADs G, H, I, N, O, Q, R, S, T, and U). Growth assay was carried out by spotting different dilutions of each K. phaffii strain on agar plates containing YNBM. Cells were grown for 72 h at 30 °C. AOX1, alcohol oxidase; 9aaTAD, nine amino acid transactivation domain; DBD, DNA-binding domain; DHAS1, dihydroxyacetone synthase ; YNBM, yeast nitrogen base and methanol.

Article Snippet: Twenty synthetic gene fragments ( TAD C 3x to TAD V 3x ) of 194 bp size encoding three tandem repeats of putative 9aaTADs C to V were synthesized by Genscript ( ).

Techniques: Functional Assay, Expressing, Sequencing, Cell Culture, SDS Page, Staining, Growth Assay, Binding Assay